Kategorie: Promotions-/PhD-Stellen

Development of innovative Multidrug Targeted Nanocarries for Imaging and Therapy of colorectal cancer

Location: 
TranslaTUM – Central Institute for Translational Cancer Research and
Department of Nuclear Medicine, Klinikum rechts der Isar München
Ismaninger Straße 22, 81675 München

Start Date:
preferably August 2022

Project Area:           
Development of innovative Multidrug Targeted Nanocarries for Imaging and Therapy of colorectal cancer. Colorectal cancer is one of the most common cancer diagnoses with almost 900.000 deaths annually. More than 50% of patients develop metastasis accompanied with poor prognosis. In this project we aim to develop a strategy for a targeted delivery of a chemotherapeutic payload to tumor cells, in order to increase therapeutic efficacy and to minimize side effects. The compounds will additionally be labeled with radionuclides and/or fluorescent dyes in order to track their biodistribution.

Your Tasks:
PhD position 1: Chemistry
–       Synthesis of cyclic peptides by solid-phase peptide synthesis
–       Synthesis of small organic molecules and conjugation
–       Development and optimization of radiolabeling procedures
–       Characterization of radiolabeled molecules

PhD position 2: Biochemistry, Biology
–       Evaluation of the radiolabeled and/or fluorescent-labeled compounds on cells and tissue samples
–       Further characterization of samples by autoradiography, microscopy and imaging flow cytometry
–       Positron emission tomography-based imaging in animal models
–       Biodistribution studies in animal models

The project will be performed by two PhD candidates who will work complementarily on separate topics. This position is a great opportunity to contribute with your own research to fight cancer. Located in the heart of Munich, our labs offer you a place in a highly motivating environment with excellent and creative working atmosphere, to perform your tasks independently and contribute with your own ideas. If you are interested and have a MSc. in Chemistry, Biochemistry, Biology or similar qualification, please send a motivation letter and CV, including your possible start date.

Contact details and further information:
PhD position 1 – Chemistry:                      Dr. Alexander Wurzer, alexander.wurzer[ät]tum.de
PhD Position 2 – Biochemistry, Biology:  Prof. Dr. Susanne Kossatz, s.kossatz[ät]tum.de

A PhD position is available in the laboratory for Cellular Protein Biochemistry at the Technical University of Munich (TUM) Department of Chemistry.

Our laboratory aims at understanding how proteins fold, assemble and are scrutinized by the cellular quality control machinery. We use an interdisciplinary approach from protein biochemistry to cell biology to analyze the machinery and mechanisms that monitor cellular protein biogenesis. One major model system in our laboratory are interleukins, key signaling molecules in our immune system. We use insights into their biogenesis to rationally engineer new immunomodulatory biomolecules. Within our translational department we then develop these proteins into next-generation biopharmaceuticals.

JOB DESCRIPTION
The ideal candidate will work with our translational team to decipher the mechanism of action of engineered immune molecules. For this, biochemical approaches in combination with molecular immunology studies will be combined. Ultimately, these insights will guide the preclinical as well as clinical development of these novel first-inclass biopharmaceuticals. With these new pharmaceuticals we aim to help patients with high-unmet medical need diseases.

RESPONSIBILITIES
• Protein expression and purification
• Biochemical and biophysical protein analytics
• Molecular immunology studies
• Ex vivo and in vivo animal studies (in collaboration)
• Translation of basic research into medical application

QUALIFICATIONS
• MSc in biochemistry or similar fields
• Expertise in protein expression, protein purification and biophysical
characterization
• Expertise in mammalian cell culture and molecular immunology is a plus
• Experience in protein engineering as well as (pre-)clinical development of
biopharmaceuticals and molecular immunology is a plus
• Know-how in GLP and GMP requirements is a plus

THE ENVIRONMENT AND THE POSITION
The position is associated with the laboratory of Cellular Protein Biochemistry (www.cell.ch.tum.de) at the Technical University of Munich (TUM), a leading research university in Europe. The salary is in accordance to the Public Sector Collective Agreement on Länder (TV-L E13/65%). The earliest starting date is September 1st, Only applications received until June 15th 2022 will be considered.
We are looking forward to your application including a CV, (degree) certificates, a letter of motivation and at least two possible references. Please send your application as a single pdf-document by email to: matthias.feige[ät]tum.de .

TUM is an equal opportunity employer. Qualified women are therefore particularly encouraged to apply. Applicants with disabilities are treated with preference given comparable qualification.

A PhD position is available in the laboratory for Cellular Protein Biochemistry at the Technical University of Munich (TUM) Department of Chemistry and Institute for Advanced Study.

Our laboratory aims at understanding how proteins fold, assemble and are scrutinized by the cellular quality control machinery. We are particularly interested in proteins of the secretory pathway – proteins that are ultimately secreted or localized on the cell surface and allow cells to interact with their environment. We use an interdisciplinary approach from protein biochemistry to cell biology to analyze the machinery and mechanisms that monitor cellular protein biogenesis.

THE PROJECT: Membrane proteins make up one third of the human proteome. They are critical for any cellular function from movement to immune defense. Membrane proteins are major drug targets and their mutations cause numerous severe human diseases. Despite these key roles in biology and medicine, cellular mechanisms of membrane protein biogenesis remain ill-defined.
This project will investigate how molecular chaperones support and control membrane protein biogenesis. It will focus on two major aspects: First, novel endoplasmic reticulum chaperones we have identified in our lab with hitherto mostly unknown functions. Second, on the question of how molecular chaperones work together on a molecular level to support and control membrane protein biogenesis.
The project thus has the potential to provide exciting insights in the molecular biology of cells. To achieve this, the project will use state-of-the-art cell biological approaches from microscopy via geneknockouts to mass spectrometry and will build on our existing collaborations with cryo-EM labs.

YOUR PROFILE: You should hold a Diploma/Master’s degree (or equivalent) in Biochemistry, Cell Biology or related fields. Previous experience in mammalian cell culture and protein biochemistry is required. Experience in gene editing techniques, protein (crosslinking) mass spectrometry and/or fluorescence microscopy is a benefit.

THE ENVIRONMENT AND THE POSITION: The Technical University of Munich (TUM) is a leading research university in Europe. We are offering excellent working conditions, participation in a structured PhD program at the TUM graduate school and an international research environment. The salary is in accordance to the Public Sector Collective Agreement on Länder (TV-L E13/65%). The position is available from August 2022 on. Only applications received until May 31st 2022 will be considered.

The application should contain a CV, (degree) certificates, a letter of motivation and at least two possible references. Please send your application as a single pdf-document by email to:
matthias.feige[ät]tum.de

TUM is an equal opportunity employer. Qualified women are therefore particularly encouraged to apply.
Applicants with disabilities are treated with preference given comparable qualification.
further information: www.cell.ch.tum.de and

PhD student (f/m/d) in functional genomics of mitochondrial stress signaling @TheJaeLab LMU Munich

Mitochondria experience substantial stress and malfunction in aging and severe human diseases, including neurodegeneration, cardiovascular defects, and cancer. Surprisingly, despite their separate genome, mitochondria do not encode any stress response genes and instead need to engage in complex interactions with the host cell to dispel any threats. Despite groundbreaking work in model organisms, how mitochondrial failure is detected, combated and prevented in the human system is largely unknown.

Using a unique functional genomics platform that combines bleeding-edge CRISPR genome engineering with ultradeep mutagenesis and phenotypic profiling (Brockmann et al., Nature, 2017), we have recently identified a pathway consisting of OMA1, DELE1 and HRI that signals a wide range of mitochondrial insults to the cytosol (Fessler et al., Nature, 2020). However, we are only beginning to understand how the central players of this pathway operate in the steady-state and in times of stress, and how this pathway interacts with other proteostasis mechanisms to maintain mitochondrial function in human cells (Eckl et al., CMLS, 2021; Fessler et al., Nature Comm. 2022).

Your profile
We are seeking highly motivated candidates who share our dedication to science and enthusiasm for interdisciplinary research with an excellent degree in biomedicine, molecular medicine, biochemistry, biology, or similar. You will plan and perform experiments of high complexity, analyze large-scale data, and work well in a team. Excellent communication skills, fluency in English, and a solid background in molecular and cellular biology techniques, as well as genetics are required. Expertise in bioinformatics and a sense of humor are a plus.

Our offer
You will be part of a young and ambitious team consisting of scientists and students of different backgrounds with a shared interest in mitochondria, proteostasis and biological questions with high relevance for human disease. You will exclusively work in the human system and be trained in cutting-edge gene editing, genome-wide screening and synthetic biology techniques, as well as classical biochemistry, cell and molecular biology applications. You will find ample opportunities for scientific exchange with world-leading scientists of various fields and develop your scientific career with lasting benefits through events on campus, seminars and hands-on courses. We are located in the new BioSys building of the Gene Center endowed with state-of-the-art laboratory space, equipment and core facilities, embedded in the vibrant, multi-disciplinary life sciences campus. Downtown Munich and the Bavarian lakes can be reached in 20min, the German Alps in 1h. The position is fully funded by the German Research Foundation (DFG) for a period of three years, with the possibility of extension. Salary is determined by prior experience according to the German public sector pay scale TV-L.

Application
Your application should consist of an informative motivation letter, CV, and transcripts of records, preferably as single pdf document, addressed to jae[ät]genzentrum.lmu.de. Applications close 1 June 2022. The University of Munich is an equal opportunity employer. Handicapped candidates with equal qualifications will be given preference.

For more information about our lab at the LMU and our research please visit our website.

Contact: Lucas Jae, jae[ät]genzentrum.lmu.de

Project Area: Structure-function analysis and cell-signaling of metastasis-promoting multi-functional protease inhibitors

Proteases and their natural inhibitors control virtually all processes of life by controlling and remodeling the proteins in the microenvironment of any cell, its receptors, as well as growth factors. Imbalance of proteases and protease inhibitors promotes detrimental inflammatory processes during infections, tumor growth, and cancer metastasis.
We study the non-canonical function of Tissue-inhibitors of metalloproteinases (TIMPs), namely their newly-discovered potential to act, unexpectedly, as a cell signaling-inducing cytokine, which triggers pro-inflammatory function in immune cells as well as other organ-resident cells. We have identified the multi-functionality of TIMP-1 and could thereby explain the paradox that TIMP-1 correlates with bad prognosis of many diseases, although its canonical anti-proteolytic activity had led to the prediction that TIMP-1 should inhibit them. We employ a wide spectrum of technologies encompassing all aspects of molecular cloning/genetic
engineering, expression-vector design, biochemistry, cell culture including functional cell-assays, metabolic assays, protein-design and purification, flow-cytometry, histology, in silico-modeling, TIMSTOF, statistical analyses etc. in order to explore new structure-function relationships between TIMPs and their receptors, which we also identify in the process.
Through cooperation with colleagues in the clinic we constantly validate our data with material from the clinic towards a transfer of new knowledge from bench to the bedside.

If you have an M.Sc. in biochemistry, (molecular) biology, molecular biotechnology or similar, and would like to work towards a PhD or Dr. rer. nat., we are happy to welcome you in our group.

Please contact me at: achim.krueger[ät]tum.de

About the Project:
Our group are interested in diverse applications of supramolecular chemistry, from coordination chemistry and self-assembly to polymer science and molecular photoswitches. For more information our group’s recent research, see https://bevesgroup.wordpress.com/publications/

This project is in collaboration with Prof. Palli Thordarson (@pallithordarson), Prof. Ayusman Sen Penn State (@ayusmansen) and Prof. Dean Astumian UMaine (@DeanAstumian).

This ARC-funded project aims to understand the mechanisms behind the directed bulk transport of molecules by controlled diffusion and flow linked by chemical reactions and chemical concentration gradients. This project will provide the first detailed experimental data to test proposed theories and produce a fundamental understanding of how molecules can undergo controlled transport. Expected outcomes include a new understanding of how molecules can be guided toward their desired targets, which could have applications in waste collection or sensing by concentrating analytes, and for understanding biological processes.

Skills gained include: Organic synthesis, host-guest chemistry, NMR spectroscopy and microfluidics experiments, optical microscopy, data analysis including kinetics modelling.

Required: BSc/MSc with Major in Chemistry; some experience with synthesis; UV-vis spectroscopy; interpreting NMR spectra. A willingness to learn some python coding and microscopy techniques, such as confocal microscopy.

PhD program: At UNSW our PhD program is 3.5 years of full time research, with no coursework requirements.

Eligibility:

· All nationalities are eligible.

· You must meet the English language requirements in order to apply. (https://www.unsw.edu.au/study/how-to-apply/english-language-requirements)

· You must have the equivalent of a 4 year undergraduate degree, with at least 9 months of research and an examined thesis (equivalent to UNSW 1^st Class Honours).

Funding Notes

The available stipends will match the Australian Government Research Training Program scholarship (2022 rate = $28,854), plus relocation expenses.

How to apply: email A/Prof. Beves your CV, academic transcript (undergraduate and any postgraduate coursework grades) and the contact details for at least two (2) referees.

Link: Supramolecular chemistry to control the transport of molecules at UNSW Sydney on FindAPhD.com

About the Project: Our group are interested in diverse applications of supramolecular chemistry, from coordination chemistry and self-assembly to polymer science and molecular photoswitches. For more information our group’s recent research, see https://bevesgroup.wordpress.com/publications/

In this project you will prepare coordination complexes and study their photophysical and electrochemical properties. From this you will understand how these can be most effectively applied to photoredox catalysis, and in multi-component molecular devices and machines. The work will involve a wide range of analytical techniques (NMR, MS, XRD, UV-vis etc) and will involve collaborations with external partners, including in ultrafast spectroscopy.

Skills gained include: Organic and inorganic chemistry synthesis, host-guest chemistry, NMR spectroscopy, optical (UV-vis and emission) spectroscopy, electrochemistry, mass spectrometry, and single crystal X-ray crystallography.

Required: BSc/MSc with Major in Chemistry; some experience with inorganic (coordination chemistry) synthesis; UV-vis spectroscopy; interpreting NMR spectra. A willingness to learn new techniques.

PhD program: At UNSW our PhD program is 3.5 years of full time research, with no coursework requirements.

Eligibility:
· You must meet the English language requirements in order to apply. (https://www.unsw.edu.au/study/how-to-apply/english-language-requirements)

· You must have the equivalent of a 4 year undergraduate degree, with at least 9 months of research and an examined thesis (equivalent to UNSW 1^st Class Honours).

Funding Notes

The available stipends will match the Australian Government Research Training Program scholarship (2022 rate = $28,854), plus relocation expenses.

How to apply: email A/Prof. Beves your CV, academic transcript (undergraduate and any postgraduate coursework grades) and the contact details for at least two (2) referees.

Link: https://www.findaphd.com/phds/project/photoredox-catalysis-by-transition-metal-complexes/?p140445

Topic: Electrophysiological investigation of the pathogenicity of genetic variants in pain associated genes of patients with small fiber pathology an.

Tasks PhD candidate:
− Electrophysiological analysis (patch-clamp, multielectrode array) of patient-derived and sensory neurons in mono- and co-culture with Schwann cells
− HEK cell transfection and electrophysiological analysis
− Generation of patient-derived Schwann cells via iPSC
− Analysis of cultured Schwann cells by immunocytochemistry, qRT-PCR, and FACS

Prerequisites: We are seeking for a very industrious, eager, and reliable PhD candidate of Life Sciences or related Faculty who is enthusiastic about scientific work, and has experience in electrophysiology (patch-clamp) and cell culture. He/she is motivated to engage himself/herself in this exciting project and to become part of our enthusiastic research team!

Constellation: Our project will be performed by two PhD candidates who will team-up and work complementarily, however, on separate topics of the entire project.

Kontakt: Prof. Dr. N. Üçeyler, ueceyler_n[ät]ukw.de.

Stellenbeschreibung:
Arbeiten Sie mit innovativen 3Mern auf der ganzen Welt.
Die Wahl des richtigen Arbeitgebers ist eine Entscheidung, die Ihr Berufs- und Privatleben gleichermaßen erheblich beeinflusst. Daher ist es umso wichtiger, sich dabei jederzeit auf die Unterstützung des Unternehmens und dessen Führungskräfte verlassen zu können. Bei 3M werden Sie Teil eines globalen Multitechnologiekonzerns, der Ihre individuellen Stärken
wertschätzt, Ihre berufliche und persönliche Weiterentwicklung fördert und Ihnen Freiraum für Kreativität, Neugier und Eigeninitiative bietet. Treiben Sie gemeinsam mit 96.000 3Mern aus verschiedenen Kulturen & Nationalitäten nachhaltige Projekte, Ideen und Innovationen voran, die eines zum Ziel haben: das tägliche Leben eines jeden Einzelnen zu verbessern.

Ihr Beitrag zu unserem gemeinsamen Erfolg:
Als Werkstudent (*) Manufacturing Technology Development am Standort Seefeld (Bayern) haben Sie die Möglichkeit Ihr Wissen zielgerichtet einzusetzen, um unseren gemeinsamen Erfolg voranzubringen. Dabei umfasst Ihr Aufgabengebiet:
• Mitarbeit bei der Optimierung und Weiterentwicklung der
vorhandenen Produktionsprozesse hinsichtlich Produktqualität und
Produktivität
• Mitarbeit bei der Entwicklung neuer Herstellverfahren
• Planung (DoE), Durchführung und Auswertung von Experimenten im Labor
• Aufbau von Versuchen (Anlagen, Equipment, Steuerungen, Sensoren, Messtechnik)
• Interpretation der Ergebnisse und Dokumentation der gewonnenen Erkenntnisse

Kontakt: Rebecca Wilhelm, rebecca.wilhelm[ät]tum.de

Short Description:
While the amount of atmospheric water harvesting (AWH) MOFs is rapidly increasing over the last decade, the technical development of analysis platforms capable of in situ studying their performance is just starting. We developed a system based on (nonlinear) laser micro- and spectroscopy, that is capable of live monitoring the effect of heterogeneity on the AWH performance on the single crystal level.

Beschreibung:
Project:
Climate warming induced draughts are an increasing problem worldwide, threatening especially poor countries. Metal-organic frameworks (MOFs) are the most promising class of materials for harvesting large quantities of water from humid air. While the amount of atmospheric water harvesting (AWH) MOFs is rapidly increasing over the last decade, the technical development of analysis platforms capable of in situ studying their performance is just starting. We developed a system based on (nonlinear) laser micro- and spectroscopy, that is capable of live monitoring the effect of heterogeneity on the AWH performance on the single crystal level.

Qualification:
We are searching for candidates with:
• Background in inorganic / physical chemistry or physics
• Interest in optical spectroscopy techniques and imaging
• Prior experience in programming (Matlab / LabView) is an asset

Our offer:
We offer a multi-disciplinary and collaborative environment for working with advanced imaging and spectroscopy on the next generation of AWH MOFs. This is your opportunity to join our team in developing an automated gas exchange system to evaluate the in-situ sorption performance of novel AWH materials.

Kontaktperson: Dr. Evelyn Ploetz, evelyn.ploetz[ät]cup.lmu.de