Kategorie: Abschlussarbeiten

Stellenangebote für Bachelor- und Master-Arbeiten

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Abschlussarbeiten Stellenangebote

Master’s thesis: Application of genetically encoded tension sensor modules to pancreatic cancer organoids

Kurzbeschreibung:
The aim of this thesis is to establish a new method for the measurement of molecular forces by applying tension sensors based on FRET to pancreatic cancer organoids. Confocal microscopy and digital imaging processing and analysis will be employed to understand how mechanical forces lead to structure formation processes in organmorphogenesis.

Your Profile:
• Surpassing grades in your master’s program in biology, (bio)chemistry, biotechnology, physics, or related scientific program
• Genuine interest in the powerful applications of cancer organoids and FLIM-FRET imaging
• Previous experience with fluorescence microscopy
• Advanced programming skills (Phython, matlab,…) for image data processing
• The ability to be self-motivated and to work independently within an interdisciplinary team of biotechnologists and physicist
• English language and organizational skills

More Information

Contact: M.Sc. Sophie Kurzbach, sophie.kurzbach[ät]tum.de

Link: https://www.bauschlab.org/

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Abschlussarbeiten Allgemein Stellenangebote

Bachelor’s thesis: Application of genetically encoded tension sensor modules to pancreatic cancer organoid

Short description:
The aim of this thesis is to establish a new method for the measurement of molecular forces by applying tension sensors based on FRET to pancreatic cancer organoids. Confocal microscopy and digital imaging processing and analysis will be employed to understand how mechanical forces lead to structure formation processes in organmorphogenesis.

Your Profile:
• Surpassing grades in your bachelor’s program in biology, (bio)chemistry, molecular biotechnology, physics, or related scientific program
• Genuine interest in the powerful applications of cancer organoids combined with FLIM-FRET imaging
• Previous experience with microscopy, fiji (imageJ) data analysis, mammalian cell culture
• The ability to be self-motivated and to work within an interdisciplinary team of biotechnologists and physicist
• English language and organizational skills

Techniques:
Mammalian cell culture, organoid culture, immunostainings, FACS, fluorescence and FLIM imaging, image processing

More Information

Contact: M.Sc. Sophie Kurzbach, sophie.kurzbach[ät]tum.de

Link: https://www.bauschlab.org/

Kategorien
Abschlussarbeiten Praktika Stellenangebote

BA / SA / MA: Potentialkontrollierte Membranchromatographie – Setup Design

Kurzbeschreibung:
Entwicklung eines neuen Setups für die Potentialkontrollierte Membranchromatographie.
Aufgaben: Entwicklung eines Membranmoduls, Simulation der Strömungseigenschaften, Inbetriebnahme an einer präparativen Chromatographieanlage
Anforderungen: Kreativität und handwerkliches Geschick, Spaß an angewandter Forschung, Von Vorteil: Erste Kenntnisse in CAD und Comsol

Beschreibung:
Ziel dieses Forschungsprojektes ist die Entwicklung eines neuartigen Chromatographieprozesses unter Einsatz von leitfähigen Membranen. Diese können in ihrer Ladung und folglich ihrem Bindecharakter beeinflusst werden und ermöglichen somit eine potentialkontrollierte Elution des Zielmoleküls. Dadurch kann die Biomolekülaufreinigung nicht nur effizienter und kostengünstiger sondern auch nachhaltiger erfolgen.
In dieser Abschlussarbeit soll ein neues Membranmodul für den Einsatz an einer präparativen Chromatographieanlage mittels CAD entworfen werden. Die Strömungseigenschaften des Moduls spielen hierbei eine entscheidende Rolle und sollen anschließend simulativ untersucht werden. In einem weiteren Schritt soll das entwickelte Modul an einer präparativen Chromatographieanlage in Betrieb genommen und auf seine Eignung untersucht werden.

Mehr Informationen

Kontaktperson: Dennis Röcker, d.roecker[ät]tum.de

Link: https://www.epe.ed.tum.de/stt/selektive-trenntechnik/

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Abschlussarbeiten Praktika Stellenangebote

Carbon capture & storage: analysis and characterization of biochar production pathways

Short description:
At the Laboratory of Chemical Process Engineering, a new project about carbon capture and storage has recently started. The overall aim of the project is to reduce the amount of carbon in the atmosphere by developing a suitable long-term carbon storage in Bavaria. There are various carbon sources, such as CO2 from air, minerals, organic waste, crops, algae and further sorts of biomass. All of them are carbon-containing resources and, therefore, must be considered as possible feedstock for carbon storage. To achieve a higher carbon content and higher density of these compounds, processes are sought to convert them to long-term carbon storage materials.
Within the scope of this project, biochar as a feasible storage material should be analyzed. Special interest applies to the characterization of different production pathways concerning yield, energy and water consumption and required temperatures for each production pathway. For more details, contact the supervisor.

Description: Good knowledge of German or English language is required, as well as interest in carbon capture and storage techniques. Priorknowledge in any of the following topics is appreciated but not required: LCA, process engineering, reaction kinetics, thermodynamics, and physical chemistry. Good support and training will be provided.

Mehr Informationen

Kontaktperson: Natalie Rosen, natalie.rosen[ät]tum.de

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Abschlussarbeiten Promotions-/PhD-Stellen Stellenangebote

Internships at Oncology Department at Novartis Institute for Biomedical Research (NIBR)

Kurzbeschreibung:
The Oncology Department of the Novartis Institute for Biomedical Research in Basel, Switzerland is currently looking for interns in the field of molecular and cellular biology. Positions are available for 6 months to work on different aspects of cancer biology.
You will be involved in cutting-edge research using the latest technologies in genetic manipulation, molecular cloning, cellular analyses and nucleic acid / protein characterization. You will join enthusiastic teams working on immune-oncology and signal transduction pathways to dissect the molecular functions of a number of candidate oncogenes/tumor suppressors in diverse cancer settings.

Beschreibung:
Your tasks will be:
– Design, execute and/or analyze experimental work with the supervision of different team members
– Contribute to lab / team organization and projects development
– Formulate and test additional hypothesis based on a large amount of internal data in different fields
– Provide accurate raw data documentation, evaluation and results interpretation

Requirements:
– M.Sc. degree in Molecular Biology (and related)
– Experience with mammalian cell culture and basic cell biology techniques (e.g. Western blotting) is required. FACS experience is preferred but not required.
– Interest / knowledge in cancer biology, immune-oncology and signal transduction are advantageous but not required.
– Good organizational skills with respect to planning, tracking and adherence to timelines
– Good communication, presentation and scientific writing skills
– Fluency in English is mandatory (oral & written)

Kontaktperson: Carlotta Costa, carlotta.costa[ät]novartis.com

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Kategorien
Abschlussarbeiten Praktika Stellenangebote

Immunological diabetes research

Short description:
1. Targeting RORγt/DHODH signaling in islet autoimmunity and Type 1 Diabetes
 2. The role of T cell-specific IL6Rα signaling in maintaining tissue immune homeostasis

Description:
1. We investigate the influence of RORγt/DHODH inhibition on Tregs and different T cell subsets in islet autoimmunity and T1D. Using various in vitro approaches and loss-of-function or T1D mouse models, we dissect the impact on Treg induction and the underlying mechanisms. In collaboration with a biopharmaceutical company, we apply drug candidates targeting these signaling pathways in vivo to different mouse models for T1D to study their effect on autoimmune activation and diabetes progression or prevention.
2. We investigate the crucial role of the high-affinity α-chain of the interleukin 6 receptor (IL6Rα) on CD4+T cells and Foxp3+ Tregs. Using different gain- and loss-of-function mouse models, we dissect the role of the IL6Rα and its complex interplay with other signaling molecules in maintaining Tregs. By that, we investigate how the IL6Rα on T cells contributes to maintaining tissue homeostasis, preventing aberrant immune activation and mediating cross-talk between tissues and immune cells which is important for the function of diabetes-relevant tissues.

More informations


Kontaktperson:
Hannah Hipp; Maike Becker – Helmholtz Zentrum München,

Email:
hannah.hipp[ät]helmholtz-muenchen.de

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Abschlussarbeiten Stellenangebote

Masterarbeit (m/f/d) Biotechnologie / Chemie beim TUM-MedTech Startup & Professur für Selektive Trenntechnik

Dymium is an early-stage MedTech startup based in Munich. Our team has developed and patented a new surgical method for the residue-free, minimal-invasive removal of kidney stone fragments using magnetism. This has the potential to save thousands of patients from follow-up surgeries every year. We are looking for a student (m/f/d) to help us with the pre-clinical testing and validation of our prototype ex vivo, i.e. in porcine (pig) kidneys.

Kontakt: shyam.sri[ät]dymium.de

Kategorien
Abschlussarbeiten Stellenangebote

Using defects in diamond for the detection of nucleic acids

Short description:
Quantum sensors in diamond are a promising platform for the detection of DNA sequences.

Description:
Recent experiments have shown that defects in diamond, in particular nitrogen-vacancy (NV) centers, can be used as quantum sensors for detecting nuclear magnetic resonance (NMR) signals from tiny volumes down to a single molecule. Our group applies this novel technology to detect chemical reactions on surfaces at the nanoscale for applications in bioanalytics.

In this interdisciplinary project, you will use NV-shallow-implanted diamond chips as a novel sensing platform for detecting nucleic acids. For this purpose, you will modify the surface of diamond chips with nucleic acids and perform NV-based magnetic resonance experiments to detect their phosphorus NMR signal. The overall goal is to develop a novel sensor for detecting DNA sequences.

Techniques: NV-NMR, biochemistry, bioassays
Skills: Ideally knowledge of NMR/EPR spectroscopies, surface chemistry, and analytical chemistry

Kontaktperson: Dominik Bucher, dominik.bucher[ät]tum.de

Link: http://www.bucherlab.org

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Abschlussarbeiten Stellenangebote

Master Thesis for targeted protein degradation project

Description: Brenda Schulman laboratory, Molecular Machines and Signaling Department, Max Planck Institute of Biochemistry

Background:
Protein modification by the small protein ubiquitin regulates nearly all aspects of eukaryotic biology.  A particular type of modification, where proteins are decorated by “chains” of ubiquitin proteins covalently linked to each other, direct proteins for degradation by the proteasome.  Defects in ubiquitin pathways are associated with diseases such as cancer, neurodegenerative disorders and viral infections. On the other hand, we can now harness the power of targeting protein degradation, by using small molecules to degrade disease causing proteins.  Targeted protein degradation is an exciting emerging area of drug development, yet the structural mechanisms of marking targeted proteins with ubiquitin remains unknown. The laboratory uses a combination of structural, cell and chemical biology, as well as biochemistry to mechanistically characterize native and drug-induced complexes in the ubiquitin-proteasome pathway.

Goal of the project:
Biochemical and structural characterization of targeted protein degradation mechanisms.

Requirements:
• General knowledge in biochemistry and molecular biology
• Ability to work in a team
• Dedication and motivation to solve important and difficult scientific questions
• Basic knowledge and experience in protein expression and purification

We offer:
• Learning of cutting-edge structural biology (cryo-EM, crystallography)
• Learning of biochemical assay development
• Participation in an interesting project
• Working at the forefront of ubiquitin field
• Excellent scientific environment
• Working with an international team of talented scientists

Application:
Please send your resumes and transcript of records to Joanna Liwocha

https://www.biochem.mpg.de/schulman

Kontaktperson: Joanna Liwocha, liwocha[ät]biochem.mpg.de

More Information
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Abschlussarbeiten Stellenangebote

Master’s Thesis: Catalytic Activation of Dinitrogen with bioinspired Complexes

Short: The overarching aim of the Mougel group research at ETH Zürich is to provide energy efficient strategies for the (electro)chemical transformation of small molecules such as nitrogen or carbon dioxide to high value-added products. Molecular catalysts allow for a high tunability of reaction active sites. We hereby take our inspiration from Nature, by aiming to mimic biological phenomena. For this work the focus of research is on synthesis, reactivity and catalytic testing of various complexes for the reduction of nitrogen.

Requirements:
– Majoring in chemistry with a focus on inorganic chemistry or catalysis
– Experience in advanced Schlenk techniques and handling the Glovebox
– Curiosity and interest in scientific problem solving
– Experience in working with IR, NMR, GC, SC-XRD or UV-Vis are beneficial

Students will learn:
– Synthesis of air sensitive ligands and complexes using Schlenk techniques
– Catalytic testing under inert conditions
– Advanced NMR and UV-VIS characterization


Application/contact:
We are looking forward to receiving your application via email to
– mstrauss[ät]ethz.ch and mougel[ät]inorg.chem.ethz.ch.
Additional application to TUM Auslandsbüro Chemie/ ETH Mobility.
Start: between February and May.

Link: https://mougel.ethz.ch/