„Analysis of cancer stemness and chemotolerance factors in colorectal cancer organoids and patient tissue samples“
Aim of the master thesis is the functional and histological characterization of cancer „stemness“ genes.
Our research group deals with the advanced progression of colorectal adenocarcinoma with a focus on liver metastasis and acquired chemotolerance, which both reflect major challenges during the clinical management of this disease. A living biobank of tumor organoids set up in our department provides an optimal basis for this research area. By performing next generation sequencing (RNA-Seq) on pre-conditioned tumor organoid lines we have identified promising candidates with functional relevance for tumor fitness/stemness and resilience towards combinatorial chemotherapy. Preliminary functional analyses have already provided solid support for our working hypotheses.
The aim of the master thesis is to conduct molecular biological and histochemical analyses, which will contribute to the characterization of factor functionality and tissue expression pattern. The candidate will have the opportunity to learn state-of-the-art methodology, such as CRISPR/Cas9-mediated genome editing and conditional miRNA expression in tumor organoid lines, quantitative chromatin-immunoprecipitation, RNA Scope in situ hybridization (Advanced Cell Diagnostics). In addition, detection of cell division rate, apoptosis, DNA and protein de novo biosynthesis via flow cytometry can be performed in the context of the project.
For this technically innovative project, which provides a deep insight into a wide spectrum of modern molecular biological methods, we are looking for a highly motivated candidate who brings a solid understanding and motivation for experimental laboratory work. Good communication skills (English and German) are beneficial.
Optimal starting date: January or February 2020
Kontaktperson: Dr. Peter Jung, email@example.com